Automated enzymatic fluorometric method for the determination of pyruvic and lactic acids in blood.
نویسنده
چکیده
A simple automated fluorometric method for the estimation of lactate and pyruvate is described. Analyses can be performed at 60/hour and can conveniently determine pyruvate and lactate in the ranges 0-5 mg. and 0-30 mg/100 ml. The methods give a high degree of analytical precision and good yields from recovery experiments.
منابع مشابه
بررسی تغییرات اسیدهای آلی در طول دوره رسیدن پنیرهای سفیر آب نمکی ایرانی با استفاده از مایه های مختلف لاکتیکی
For Iranian fermented cheese processing and ripening, different lactic acid bacteria (LAB) that affect on the physicochemical properties and hence the organoleptic characteristics of the cheese is used. Determination of physicochemical changes of cheese, particularly, organic acids is of importance. In this study five cheese formulas with five different group of cheese starters were processed a...
متن کاملبررسی تغییرات اسیدهای آلی در طول دوره رسیدن پنیرهای سفیر آب نمکی ایرانی با استفاده از مایه های مختلف لاکتیکی
For Iranian fermented cheese processing and ripening, different lactic acid bacteria (LAB) that affect on the physicochemical properties and hence the organoleptic characteristics of the cheese is used. Determination of physicochemical changes of cheese, particularly, organic acids is of importance. In this study five cheese formulas with five different group of cheese starters were processed a...
متن کاملNew Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots
Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...
متن کاملAutomated fluorometric determination of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities in red blood cells.
An automated fluorometric microtechnic, suitable for screening purposes, has been developed for the quantitative determination of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) levels in red blood cells. The method is rapid, simple, and precise. It uses 50 p.I whole-blood (cpillary) samples and is based on the measurement of natural fluorescence emitted by ...
متن کاملAn enzymatic-spectrophotometric determination of pyruvic and lactic acid in blood. Methodologic aspects.
1N11 ANY clinical and experimental problems concerning anaerobic metabolism and the accumulation of an oxygen debt have recently been studied, using Huckabee’s concept of “excess lactate” (1). Colonmetric methods have most often been used to measure pyruvic and lactic acid in blood (2, 3). The estimation of these two metabolites by an enzymatic analysis has advantages-e.g., specificity and ease...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 21 2 شماره
صفحات -
تاریخ انتشار 1968